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UNIQUE ACCESSION NUMBERS, ORDERABLES, ORDERSETS & TIME STUDIES
1. Lavender Microtainer®: A non-evacuated tube for collection of very small volume blood samples where a lavender top tube is required. Specimen is usually collected by the capillary puncture technique, though venipunture may be used.
Volume: 2.5-6.0 µL
Anticoagulant: K2EDTA (ethylenediamine tetraacetate)
Anticoagulant mechanism of action: a chelating agent that binds calcium, forming insoluble calcium salts.
Tube purposes: Platelet counts. Complete Blood Counts (CBC) and blood film preparation for cell differentiation.
Other tests requiring very small volume samples. Consult the laboratory.
2. Lt. Blue Top: (Citrate tube)
Volumes: 2.7 mL and 4.5 mL draws
Anticoagulant: Sodium Citrate
Anticoagulant method of action: a chelating agent that binds calcium, forming insoluble calcium salts.
Tube purposes:
Used for all coagulation determinations and factor assays.
Used for platelet counts when EDTA tube platelet results display aberrant phenomenon such as platelet satelliting.
Pseudonyms: Blue top, citrate tube, coag tube
NOTE: These tubes must be filled at least to the blue line on the paper label. Since the anticoagulant is in liquid form there is a dilution factor that must be maintained for accurate and reproducible result.
3. Serum Separator Tube ( SST® ) by Becton, Dickinson: Specialized "clot" tube, used to isolate serum from suspended blood cells.
Stopper color: Red/Gray (described as speckled or camouflaged
Volumes: 4.0 mL, 9.5 mL and Microtainer® for capillary punctures
Additives:
Polymer Gel: upon centrifugation, this gelatin forms a barrier between the cell and serum layers, thus making it easier to pour-off and reducing reactivity between cell and serum components.
Silica activator: Increases speed of clot formation.
Tube purposes: With only a few exceptions, can be used whenever serum is required for testing.
NEVER draw this tube for Blood Bank use, since both serum and cells are required for performing testing.
A very limited number of highly specialized therapeutic drug levels may require the use of plain red top tubes.
Pseudonyms: Tiger top, SST, Corvac™, Separator tube, speckled top, camouflaged tube.
4. Plain Red Top (Silicone-coated interior):
Stopper color: plain red
Volume: 7 mL draw
Tube additives: none (silicone-coating)
Tube purpose: General serum tube
MUST be used for Blood Banking.
May be used whenever serum is required for testing.
Pseudonym: Clot Tube, Plain Red
5. Green Top:
Volume: 3.0 mL draw, 10.0 mL draw, Microtainer® for capillary punctures available.
Anticoagulant: freeze dried Sodium Heparin
Also available with ammonium heparin or lithium heparin anticoagulants. University Hospital does not use tubes with these additives.
Anticoagulant mechanism of action: inactivates thrombin and thromboplastin.
Tube purpose: plasma determinations such as ammonia, ionized calcium and plasma hemoglobin. Also used for tissue typing and cytogenetic determinations.
6. Lavender Top:
Volumes: 3.0 mL draw, 5.0 mL draw, *Microtainer®: for capillary punctures
Anticoagulant: K2EDTA (ethylenediamine tetraacetate)
Anticoagulant mechanism of action: a chelating agent that binds calcium, forming insoluble calcium salts.
Tube purposes:
Platelet counts and platelet function studies due to EDTA's ability to inhibit platelet aggregation.
Complete Blood Counts (CBC) and preparation of blood films for cell differentiation.
Miscellaneous testing such as cyclosporin levels, hemoglobin A1C, viral load testing, sickle cell testing, sedimentation rates and others.
7. Yellow Top: (ACD)
Volumes:
Solution A: 8.5 mL draw
Solution B: 3.0 mL draw and 6.0 mL draw
Anticoagulant:
ACD = Acid-Citrate-Dextrose
Solution A: trisodium citrate, 22.0 g/L; citric acid, 8.0 g/L; and dextrose, 24.5 g/L
Solution B: trisodium citrate, 13.2 g/L; citric acid, 4.8 g/L; and dextrose, 14.7 g/L
Tube purposes: Primarily for use in blood bank studies, HLA phenotyping, DNA and Paternity testing.
8. Red Microtainer® A non-evacuated tube for collection of very small volume blood samples where a red top tube is required. Specimen is usually collected by the capillary puncture technique, though venipunture may be used.
Volume: 2.5-6.0 µL
Additives:
Polymer Gel: upon centrifugation, this gelatin forms a barrier between the cell and serum layers, thus making it easier to pour-off and reducing reactivity between cell and serum components.
Silica activator: Increases speed of clot formation.
Tube purposes:
small volume testing where serum is required
DO NOT use for Blood Bank testing
Type of containers:
Sodium heparin .
For lead determinations. This tube is certified to contain less than .01 µg/ml (ppm) lead. Tube inversions prevent clotting.
Red cap - Plain tube
For serum determinations in chemistry, serology and blood banking.
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The type of container may also viewed in the 'Shortcut To His-User(S)' (on your desk top)
Which container to use? View container type in "Specimen Log In"
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For plasma determinations in chemistry. Tube inversions prevent clotting.
Lithium heparin and gel
for plasma separation
view e-amil
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Flouride Oxalate
For glucose determinations. Tube inversions ensure proper mixing of additive and blood. Oxalate and heparin, anticoagulants, will give plasma samples. Without them, samples are serum.
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Liquid K3EDTA
Spray-dried K3EDTA
For whole blood hematology determinations. Tube inversions prevent clotting.
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105M Sodium citrate
(3.2%)
.129M Sodium citrate
(3.8%)
For coagulation determinations on plasma specimens. Tube inversions prevent clotting. NOTE: Certain tests require chilled specimens. Follow recommended procedures for collection and transport of specimen.
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Feel free to e-mail me at sehomar@hotmail.com
All enquiries are most welcome.
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